General Lab Techniques MCQs

1. Which of the following is NOT a basic safety precaution in the laboratory? A) Wearing appropriate personal protective equipment B) Eating and drinking in the lab C) Understanding emergency procedures D) Properly labeling chemicals and samples Answer: B) Eating and drinking in the lab 2. What is the purpose of a fume hood in a laboratory? A) To store chemicals B) To dispose of waste C) To provide ventilation for hazardous fumes D) To sterilize equipment Answer: C) To provide ventilation for hazardous fumes 3. Which type of glassware is typically used for precise volume measurements? A) Beaker B) Test tube C) Graduated cylinder D) Erlenmeyer flask Answer: C) Graduated cylinder 4. What does the acronym MSDS stand for in laboratory safety? A) Material Safety Data Sheet B) Medical Safety Data Sheet C) Lab Equipment Data Sheet D) Material Storage Data Sheet Answer: A) Material Safety Data Sheet 5. Which instrument is used to measure the mass of an object? A) Thermometer B) Balance C) Pipette D) Spectrophotometer Answer: B) Balance 6. What does the term “aseptic technique” refer to in the lab? A) Keeping the lab clean B) Sterilizing equipment C) Handling materials in a way that prevents contamination D) Using precise measurements Answer: C) Handling materials in a way that prevents contamination 7. What is the purpose of a centrifuge in a laboratory? A) To measure pH B) To mix solutions C) To separate components based on density D) To measure volume Answer: C) To separate components based on density 8. What is the function of a Bunsen burner in a laboratory? A) To measure temperature B) To sterilize equipment C) To provide a flame for heating D) To measure pH Answer: C) To provide a flame for heating 9. Which type of pipette is designed for measuring and transferring small volumes of liquids? A) Volumetric pipette B) Serological pipette C) Micropipette D) Mohr pipette Answer: C) Micropipette 10. What is the purpose of calibration in laboratory instruments? A) To sterilize the instrument B) To adjust the instrument to a standard reference C) To clean the instrument D) To measure volume Answer: B) To adjust the instrument to a standard reference 11. Which of the following is NOT a component of a microscope? A) Eyepiece B) Objective lens C) Stage D) Pipette Answer: D) Pipette 12. What does the acronym ELISA stand for in laboratory testing? A) Enzyme-Linked Inflammation System Analysis B) Enzyme-Linked Immunoblotting Assay C) Enzyme-Linked Immunosorbent Assay D) Enzyme-Linked Isolation Sequence Analysis Answer: C) Enzyme-Linked Immunosorbent Assay 13. What is the purpose of agarose gel electrophoresis in the lab? A) To measure pH B) To separate DNA fragments based on size C) To sterilize equipment D) To measure volume Answer: B) To separate DNA fragments based on size 14. Which of the following is used to measure the pH of a solution? A) Thermometer B) pH meter C) Spectrophotometer D) Balance Answer: B) pH meter 15. What does the term “incubation” refer to in laboratory procedures? A) Storing samples at a controlled temperature B) Mixing solutions C) Measuring volume D) Sterilizing equipment Answer: A) Storing samples at a controlled temperature 16. Which of the following is NOT a step in the scientific method? A) Hypothesis B) Conclusion C) Observation D) Repetition Answer: D) Repetition 17. What is the purpose of a spectrophotometer in the lab? A) To measure temperature B) To measure pH C) To analyze the concentration of a substance in a solution D) To sterilize equipment Answer: C) To analyze the concentration of a substance in a solution 18. Which of the following is NOT a common laboratory solvent? A) Water B) Ethanol C) Chloroform D) Salt Answer: D) Salt 19. What is the purpose of a hot plate in the laboratory? A) To measure temperature B) To sterilize equipment C) To provide heat for reactions D) To measure pH Answer: C) To provide heat for reactions 20. What does the acronym PCR stand for in molecular biology? A) Polymerase Chain Reaction B) Protein Concentration Regulation C) Plasmid Cleavage Reaction D) Protein Chromatography Resolution Answer: A) Polymerase Chain Reaction 21. Which of the following is used to measure absorbance in a spectrophotometer? A) Fluorescence B) Light intensity C) Voltage D) Resistance Answer: B) Light intensity 22. What is the function of a magnetic stirrer in the laboratory? A) To measure temperature B) To mix solutions using a magnetic field C) To measure pH D) To sterilize equipment Answer: B) To mix solutions using a magnetic field 23. What does the term “titration” refer to in laboratory experiments? A) Measuring temperature B) Mixing solutions C) Determining the concentration of a substance in a solution D) Sterilizing equipment Answer: C) Determining the concentration of a substance in a solution 24. Which of the following is NOT a type of chromatography technique? A) Gas chromatography B) Liquid chromatography C) Column chromatography D) Magnetic chromatography Answer: D) Magnetic chromatography 25. What does the acronym HPLC stand for in analytical chemistry? A) High-Performance Liquid Chromatography B) High-Pressure Liquid Concentration C) High-Precision Laboratory Calibration D) High-Potential Liquid Chemicals Answer: A) High-Performance Liquid Chromatography 26. Which of the following is NOT a type of microscope? A) Electron microscope B) Light microscope C) Scanning probe microscope D) Thermometer microscope Answer: D) Thermometer microscope 27. What is the primary purpose of a desiccator in a laboratory? A) To store biological samples B) To measure temperature C) To maintain a dry environment for storing hygroscopic materials D) To sterilize equipment Answer: C) To maintain a dry environment for storing hygroscopic materials 28. What does the term “centrifugation” refer to in laboratory procedures? A) Heating samples to a high temperature B) Separating components of a mixture based on density using centrifugal force C) Measuring the pH of a solution D) Mixing solutions using a centrifuge Answer: B) Separating components of a mixture based on density using centrifugal force 29. Which of the following is NOT a type of biological safety cabinet? A) Class I B) Class II C) Class III D) Class IV Answer: D) Class IV 30. What is the function of an autoclave in a laboratory? A) To measure temperature B) To sterilize equipment using steam under pressure C) To measure pH D) To mix solutions Answer: B) To sterilize equipment using steam under pressure 31. Which type of pipette is designed for delivering a fixed volume of liquid? A) Volumetric pipette B) Serological pipette C) Mohr pipette D) Pasteur pipette Answer: A) Volumetric pipette 32. What does the acronym SOP stand for in laboratory protocols? A) Standard Operating Procedure B) Safety Observation Protocol C) Scientific Observation Procedure D) Sample Observation Protocol Answer: A) Standard Operating Procedure 33. Which of the following is NOT a common method of sterilizing laboratory equipment? A) Autoclaving B) Filtration C) Boiling in water D) Exposure to ultraviolet (UV) light Answer: C) Boiling in water 34. What is the purpose of a water bath in the laboratory? A) To measure temperature B) To sterilize equipment C) To provide a controlled temperature environment for samples D) To measure pH Answer: C) To provide a controlled temperature environment for samples 35. What does the term “pipetting” refer to in laboratory procedures? A) Measuring temperature B) Transferring liquids using a pipette C) Measuring pH D) Sterilizing equipment Answer: B) Transferring liquids using a pipette 36. Which of the following is NOT a common hazard in the laboratory? A) Chemical spills B) Electric shock C) Loud noises D) Excessive humidity Answer: D) Excessive humidity 37. What is the function of a reagent in a laboratory experiment? A) To measure pH B) To provide a specific chemical reaction C) To mix solutions D) To measure temperature Answer: B) To provide a specific chemical reaction 38. Which of the following is NOT a type of laboratory glassware? A) Beaker B) Test tube C) Centrifuge D) Flask Answer: C) Centrifuge 39. What does the acronym TLC stand for in chromatography? A) Thin-Layer Chromatography B) Total Liquid Chromatography C) Temperature-Limited Chromatography D) Testable Liquid Chromatography Answer: A) Thin-Layer Chromatography 40. What is the purpose of a biosafety cabinet? A) To protect the environment from harmful biological agents B) To sterilize equipment C) To measure pH D) To provide a controlled temperature environment Answer: A) To protect the environment from harmful biological agents

General Lab Techniques MCQs

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