Biotechnology — MCQs August 22, 2025 by u930973931_answers 50 Score: 0 Attempted: 0/50 Subscribe 1. Which of the following is known as the father of genetic engineering? (A) Herbert Boyer (B) Paul Berg (C) Stanley Cohen (D) Watson and Crick 2. The term “biotechnology” was first coined by: (A) Karl Ereky (B) Louis Pasteur (C) Gregor Mendel (D) Robert Hooke 3. Which technique is used to amplify DNA fragments? (A) Gel electrophoresis (B) Polymerase Chain Reaction (PCR) (C) DNA sequencing (D) Microarray 4. Golden rice is genetically modified to contain high amounts of: (A) Iron (B) Vitamin A (C) Vitamin C (D) Protein 5. Which bacterium is widely used in genetic engineering for plant transformation? (A) Escherichia coli (B) Bacillus subtilis (C) Agrobacterium tumefaciens (D) Pseudomonas putida 6. Restriction enzymes are also called: (A) Ligases (B) Endonucleases (C) Polymerases (D) Exonucleases 7. First cloned mammal was: (A) Cow (B) Sheep (Dolly) (C) Mouse (D) Goat 8. The first recombinant DNA was created in: (A) 1969 (B) 1972 (C) 1975 (D) 1980 9. Which is an example of ex-situ conservation? (A) Biosphere reserves (B) National parks (C) Botanical gardens (D) Sacred groves 10. Which of the following is used as a vector in human gene therapy? (A) Bacteriophage (B) Adenovirus (C) Plasmid (D) Retrovirus 11. The Human Genome Project was completed in: (A) 1990 (B) 2000 (C) 2003 (D) 2005 12. The CRISPR-Cas9 system is used for: (A) Protein synthesis (B) DNA sequencing (C) Genome editing (D) Cloning 13. Bt cotton is resistant to: (A) Viral infections (B) Fungal diseases (C) Insect pests (D) Herbicides 14. Which of the following is used for insulin production by recombinant DNA technology? (A) Streptococcus (B) Escherichia coli (C) Bacillus subtilis (D) Agrobacterium 15. The first therapeutic product made using recombinant DNA was: (A) Growth hormone (B) Insulin (C) Interferon (D) Hepatitis vaccine 16. Plasmids are: (A) Chromosomes (B) Small circular DNA molecules (C) RNA fragments (D) Enzymes 17. In gel electrophoresis, DNA fragments move towards the: (A) Cathode (B) Anode (C) Both electrodes (D) Remain in place 18. Which of the following crops is called “GM mustard” in India? (A) Dhara Mustard Hybrid-11 (DMH-11) (B) Pusa Bold (C) Varuna (D) NRCHB-101 19. Bioreactors are used for: (A) DNA isolation (B) Large-scale production of products (C) Genetic mapping (D) Gene sequencing 20. Which of the following is NOT a product of biotechnology? (A) Antibiotics (B) Vaccines (C) Plastics (D) Insulin 21. Which method is used for DNA fingerprinting? (A) RFLP (B) PCR (C) Southern blotting (D) All of these 22. Somatic cell nuclear transfer (SCNT) was used in the cloning of: (A) E. coli (B) Dolly the sheep (C) Bt cotton (D) Golden rice 23. Micropropagation is a technique of: (A) Animal breeding (B) Plant tissue culture (C) DNA sequencing (D) Protein purification 24. Which of the following is NOT a transgenic animal? (A) Oncomouse (B) Dolly (C) Sheep producing human protein (D) None of these 25. Which technique separates proteins based on size? (A) Chromatography (B) Western blotting (C) SDS-PAGE (D) PCR 26. Which of the following is used as a molecular scissor? (A) DNA ligase (B) DNA polymerase (C) Restriction endonuclease (D) RNA polymerase 27. A hybridoma cell is produced by fusion of: (A) T-cell and B-cell (B) Tumor cell and B-cell (C) Stem cell and Tumor cell (D) None of these 28. The edible vaccine is developed in: (A) Banana (B) Tomato (C) Potato (D) All of these 29. Which process uses microorganisms to degrade harmful pollutants? (A) Bioremediation (B) Biomagnification (C) Bioprospecting (D) Bioleaching 30. DNA sequencing method developed by Frederick Sanger is called: (A) Chain termination method (B) Maxam–Gilbert method (C) Shotgun method (D) CRISPR method 31. The first genetically modified crop was: (A) Bt Cotton (B) Flavr Savr Tomato (C) Golden Rice (D) Soybean 32. Bioinformatics deals with: (A) Collecting fossils (B) Protein folding study (C) Storing and analyzing biological data (D) Identifying diseases 33. Which is an example of an enzyme used in PCR? (A) Ligase (B) Taq polymerase (C) Endonuclease (D) Helicase 34. Which of the following is NOT an application of biotechnology? (A) Gene therapy (B) Stem cell culture (C) Fossil dating (D) Vaccine production 35. In cloning, the donor nucleus is taken from a: (A) Somatic cell (B) Gamete (C) Zygote (D) Embryo 36. Single cell protein (SCP) is obtained from: (A) Algae (B) Fungi (C) Bacteria (D) All of these 37. The genome editing tool CRISPR was adapted from: (A) Human immune system (B) Plant genome (C) Bacterial defense system (D) Viral DNA 38. Which of the following is an example of biofuel? (A) Diesel (B) Biodiesel (C) Petrol (D) Kerosene 39. The process of introducing foreign DNA into a host cell is called: (A) Replication (B) Transformation (C) Transduction (D) Transcription 40. Who discovered the structure of DNA? (A) Rosalind Franklin (B) Watson and Crick (C) Mendel (D) Paul Berg 41. Which of the following is a proteomics technique? (A) SDS-PAGE (B) 2D gel electrophoresis (C) Mass spectrometry (D) All of these 42. Which organism is called “the workhorse of biotechnology”? (A) Yeast (B) Escherichia coli (C) Bacillus subtilis (D) Agrobacterium 43. Which is an example of in-situ conservation? (A) Gene banks (B) Cryopreservation (C) National parks (D) Seed banks 44. Which is an advantage of tissue culture? (A) Disease-free plants (B) Rapid multiplication (C) Year-round production (D) All of these 45. The enzyme reverse transcriptase synthesizes: (A) RNA from DNA (B) DNA from RNA (C) Protein from RNA (D) DNA from DNA 46. Which of the following is a transgenic fish? (A) GloFish (B) Tilapia (C) Salmon (D) Tuna 47. DNA ligase is used to: (A) Cut DNA (B) Join DNA fragments (C) Replicate DNA (D) Sequence DNA 48. Bioethanol is commonly produced from: (A) Sugarcane (B) Wheat (C) Corn (D) All of these 49. Which of the following is NOT a component of PCR? (A) DNA template (B) Primers (C) Restriction enzyme (D) Taq polymerase 50. Which of the following is called “molecular glue”? (A) DNA ligase (B) Helicase (C) Polymerase (D) Endonuclease