Biochemistry — MCQs August 22, 2025 by u930973931_answers 50 Score: 0 Attempted: 0/50 Subscribe 1. The building blocks of proteins are: (A) Nucleotides (B) Amino acids (C) Fatty acids (D) Monosaccharides 2. The genetic material in most living organisms is: (A) RNA (B) DNA (C) Protein (D) Lipids 3. The main energy currency of the cell is: (A) NADH (B) ATP (C) GTP (D) FADH₂ 4. The monomer unit of nucleic acids is: (A) Monosaccharide (B) Amino acid (C) Nucleotide (D) Fatty acid 5. The process of converting glucose to pyruvate is known as: (A) Glycolysis (B) Gluconeogenesis (C) Krebs cycle (D) Beta oxidation 6. The enzyme that synthesizes DNA is: (A) DNA polymerase (B) RNA polymerase (C) DNA ligase (D) Primase 7. The enzyme that catalyzes the synthesis of RNA from DNA is: (A) RNA polymerase (B) Reverse transcriptase (C) DNA polymerase (D) Ligase 8. The bond between amino acids in proteins is called: (A) Hydrogen bond (B) Peptide bond (C) Glycosidic bond (D) Phosphodiester bond 9. The essential amino acid is: (A) Glycine (B) Alanine (C) Lysine (D) Glutamate 10. The vitamin that acts as a cofactor in blood clotting is: (A) Vitamin A (B) Vitamin K (C) Vitamin C (D) Vitamin D 11. Hemoglobin is an example of a: (A) Enzyme (B) Hormone (C) Transport protein (D) Structural protein 12. The enzyme responsible for lactose digestion is: (A) Sucrase (B) Amylase (C) Lactase (D) Maltase 13. The secondary structure of proteins is stabilized by: (A) Ionic bonds (B) Peptide bonds (C) Hydrogen bonds (D) Disulfide bonds 14. The TCA cycle occurs in the: (A) Cytoplasm (B) Nucleus (C) Mitochondria (D) Ribosomes 15. The end product of glycolysis is: (A) Acetyl-CoA (B) Lactate (C) Pyruvate (D) Glucose-6-phosphate 16. The bond between nucleotides in DNA is: (A) Peptide bond (B) Hydrogen bond (C) Phosphodiester bond (D) Ionic bond 17. The structure of DNA was proposed by: (A) Darwin and Wallace (B) Watson and Crick (C) Franklin and Chargaff (D) Avery and MacLeod 18. The enzyme that unwinds DNA is: (A) Ligase (B) Helicase (C) Topoisomerase (D) Polymerase 19. The primary storage polysaccharide in animals is: (A) Cellulose (B) Glycogen (C) Starch (D) Pectin 20. The primary storage polysaccharide in plants is: (A) Glycogen (B) Starch (C) Cellulose (D) Inulin 21. The vitamin required for collagen synthesis is: (A) Vitamin B1 (B) Vitamin C (C) Vitamin K (D) Vitamin E 22. The first enzyme in glycolysis is: (A) Hexokinase (B) Phosphofructokinase (C) Pyruvate kinase (D) Aldolase 23. The enzyme responsible for replication of RNA from an RNA template is: (A) Reverse transcriptase (B) RNA replicase (C) DNA polymerase (D) Ligase 24. The pH at which a protein has no net charge is called: (A) pKa (B) Isoelectric point (C) Equilibrium point (D) Dissociation constant 25. The amino acid that contains a sulfur atom is: (A) Serine (B) Cysteine (C) Glycine (D) Proline 26. The enzyme that synthesizes ATP in oxidative phosphorylation is: (A) ATPase (B) ATP synthase (C) Kinase (D) Dehydrogenase 27. The genetic code is: (A) Overlapping (B) Non-overlapping (C) Ambiguous (D) Species specific 28. Enzymes are mainly composed of: (A) Lipids (B) Proteins (C) Carbohydrates (D) Nucleic acids 29. The enzyme that joins Okazaki fragments is: (A) DNA polymerase (B) Primase (C) DNA ligase (D) Helicase 30. A holoenzyme consists of: (A) Enzyme only (B) Apoenzyme + Cofactor (C) Cofactor only (D) Substrate + Enzyme 31. The vitamin B12 contains: (A) Iron (B) Magnesium (C) Cobalt (D) Zinc 32. The oxygen-carrying pigment in red blood cells is: (A) Chlorophyll (B) Hemoglobin (C) Myoglobin (D) Bilirubin 33. The enzyme that converts pyruvate to acetyl-CoA is: (A) Pyruvate carboxylase (B) Pyruvate dehydrogenase (C) Lactate dehydrogenase (D) Pyruvate kinase 34. The enzyme needed for transcription initiation is: (A) DNA ligase (B) Sigma factor (C) DNA polymerase (D) Helicase 35. The vitamin essential for vision is: (A) Vitamin A (B) Vitamin D (C) Vitamin K (D) Vitamin C 36. Enzyme activity is affected by: (A) Temperature (B) pH (C) Substrate concentration (D) All of the above 37. The functional unit of heredity is: (A) Chromosome (B) Gene (C) Protein (D) DNA 38. The energy yield of complete oxidation of one glucose molecule is approximately: (A) 12 ATP (B) 24 ATP (C) 36 ATP (D) 48 ATP 39. The vitamin that prevents scurvy is: (A) Vitamin A (B) Vitamin B12 (C) Vitamin C (D) Vitamin D 40. The enzyme that converts fibrinogen to fibrin in blood clotting is: (A) Trypsin (B) Thrombin (C) Plasmin (D) Renin 41. The coding sequence in DNA is known as: (A) Exon (B) Intron (C) Promoter (D) Enhancer 42. The non-coding sequence in DNA is known as: (A) Exon (B) Intron (C) Operon (D) Codon 43. The coenzyme derived from niacin is: (A) NAD⁺ (B) FAD (C) CoA (D) FMN 44. The coenzyme derived from riboflavin is: (A) NAD⁺ (B) FAD (C) TPP (D) Biotin 45. The prosthetic group in cytochromes is: (A) Iron-sulfur cluster (B) Heme (C) Cobalt (D) Magnesium 46. The terminal electron acceptor in the electron transport chain is: (A) NADH (B) FAD (C) Oxygen (D) ATP 47. The enzyme used in PCR is: (A) DNA ligase (B) Taq polymerase (C) Reverse transcriptase (D) RNA polymerase 48. Which bond stabilizes the double helix structure of DNA? (A) Ionic bond (B) Peptide bond (C) Hydrogen bond (D) Phosphodiester bond 49. The one-carbon metabolism involves which vitamin? (A) Biotin (B) Folate (C) Vitamin A (D) Vitamin K 50. The enzyme responsible for DNA proofreading is: (A) DNA polymerase (B) RNA polymerase (C) Helicase (D) Primase