1. What is the primary purpose of residue analysis in food science?
A) To determine the nutritional content
B) To detect the presence of pesticides and contaminants
C) To evaluate the taste and aroma
D) To measure the moisture content
Answer: B) To detect the presence of pesticides and contaminants
2. Which analytical technique is commonly used to detect pesticide residues in food?
A) Gas Chromatography (GC)
B) Spectrophotometry
C) Atomic Absorption Spectroscopy (AAS)
D) Electrophoresis
Answer: A) Gas Chromatography (GC)
3. What is the role of Liquid Chromatography (LC) in residue analysis?
A) To separate volatile compounds
B) To quantify mineral content
C) To separate non-volatile compounds
D) To measure pH levels
Answer: C) To separate non-volatile compounds
4. Which method is used to confirm the presence of heavy metals in food?
A) Mass Spectrometry (MS)
B) High-Performance Liquid Chromatography (HPLC)
C) Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
D) Fluorescence Spectroscopy
Answer: C) Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
5. What does the term ‘limit of detection’ (LOD) refer to in residue analysis?
A) The maximum concentration that can be detected
B) The minimum concentration that can be detected reliably
C) The threshold for regulatory compliance
D) The highest permissible level of contamination
Answer: B) The minimum concentration that can be detected reliably
6. Which of the following is a common pesticide residue found in fruits?
A) DDT
B) Asbestos
C) Lead
D) Cadmium
Answer: A) DDT
7. Which regulatory body sets limits for pesticide residues in food in the United States?
A) Environmental Protection Agency (EPA)
B) Food and Drug Administration (FDA)
C) Department of Agriculture (USDA)
D) Centers for Disease Control and Prevention (CDC)
Answer: A) Environmental Protection Agency (EPA)
8. What is the significance of using a matrix-matched calibration in residue analysis?
A) It ensures the calibration standard is prepared in the same matrix as the sample
B) It simplifies the analysis process
C) It increases the sensitivity of the detection method
D) It eliminates the need for sample preparation
Answer: A) It ensures the calibration standard is prepared in the same matrix as the sample
9. Which technique is used for the analysis of mycotoxins in food products?
A) Gas Chromatography-Mass Spectrometry (GC-MS)
B) Nuclear Magnetic Resonance (NMR)
C) UV-Visible Spectroscopy
D) X-ray Diffraction (XRD)
Answer: A) Gas Chromatography-Mass Spectrometry (GC-MS)
10. What is the purpose of sample homogenization in residue analysis?
A) To improve the accuracy of the measurement
B) To increase the concentration of contaminants
C) To prepare the sample for extraction
D) To remove the contaminants
Answer: C) To prepare the sample for extraction
11. Which type of contamination is most likely to be identified using Atomic Absorption Spectroscopy (AAS)?
A) Organic contaminants
B) Heavy metals
C) Pesticide residues
D) Mycotoxins
Answer: B) Heavy metals
12. What is a common sample preparation technique for residue analysis?
A) Sifting
B) Filtration
C) Acid digestion
D) Boiling
Answer: C) Acid digestion
13. Which method is used for the detection of residual antibiotics in food?
A) Enzyme-Linked Immunosorbent Assay (ELISA)
B) Gas Chromatography (GC)
C) Inductively Coupled Plasma (ICP)
D) High-Performance Liquid Chromatography (HPLC)
Answer: A) Enzyme-Linked Immunosorbent Assay (ELISA)
14. Which of the following is a common contaminant in dairy products?
A) Dioxins
B) Asbestos
C) PCBs
D) Mercury
Answer: C) PCBs
15. In residue analysis, what does the term ‘recovery’ refer to?
A) The amount of contaminant removed during extraction
B) The percentage of contaminant detected in the sample
C) The efficiency of the analytical method
D) The amount of contaminant returned to the sample
Answer: B) The percentage of contaminant detected in the sample
16. Which technique is used for the analysis of volatile organic compounds in food?
A) Mass Spectrometry (MS)
B) Gas Chromatography (GC)
C) Fourier Transform Infrared Spectroscopy (FTIR)
D) Differential Scanning Calorimetry (DSC)
Answer: B) Gas Chromatography (GC)
17. Which type of contamination is analyzed using High-Performance Liquid Chromatography (HPLC)?
A) Radioactive isotopes
B) Heavy metals
C) Organic contaminants
D) Microbial contaminants
Answer: C) Organic contaminants
18. What does ‘quantitative analysis’ in residue analysis mean?
A) Identifying the presence of contaminants
B) Determining the concentration of contaminants
C) Measuring the taste of contaminants
D) Testing for the absence of contaminants
Answer: B) Determining the concentration of contaminants
19. Which of the following is used to assess the risk of pesticide residues in food?
A) Toxicity profiling
B) Sensory evaluation
C) Nutritional analysis
D) Colorimetry
Answer: A) Toxicity profiling
20. What is the purpose of ‘clean-up’ procedures in residue analysis?
A) To remove interference from the sample matrix
B) To concentrate the sample
C) To homogenize the sample
D) To calibrate the analytical instruments
Answer: A) To remove interference from the sample matrix
21. What type of analysis is used to detect aflatoxins in food products?
A) Enzyme-Linked Immunosorbent Assay (ELISA)
B) Gas Chromatography (GC)
C) Atomic Absorption Spectroscopy (AAS)
D) Nuclear Magnetic Resonance (NMR)
Answer: A) Enzyme-Linked Immunosorbent Assay (ELISA)
22. Which analytical technique is known for its high sensitivity in detecting trace levels of contaminants?
A) Gas Chromatography-Mass Spectrometry (GC-MS)
B) High-Performance Liquid Chromatography (HPLC)
C) Fourier Transform Infrared Spectroscopy (FTIR)
D) Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES)
Answer: A) Gas Chromatography-Mass Spectrometry (GC-MS)
23. What is the main challenge in detecting low-level pesticide residues in food?
A) High cost of analysis
B) Complexity of sample preparation
C) Sensitivity and specificity of the analytical method
D) Regulatory limits
Answer: C) Sensitivity and specificity of the analytical method
24. What does ‘precision’ refer to in residue analysis?
A) The accuracy of the measurement
B) The repeatability of the measurement
C) The limit of detection
D) The calibration range
Answer: B) The repeatability of the measurement
25. Which of the following methods is used for the detection of PCB residues?
A) Gas Chromatography-Mass Spectrometry (GC-MS)
B) Atomic Absorption Spectroscopy (AAS)
C) Enzyme-Linked Immunosorbent Assay (ELISA)
D) Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
Answer: A) Gas Chromatography-Mass Spectrometry (GC-MS)
26. What is the purpose of a ‘blank’ sample in residue analysis?
A) To calibrate the instrument
B) To check for contamination during the analysis
C) To measure the recovery rate
D) To prepare calibration standards
Answer: B) To check for contamination during the analysis
27. Which substance is commonly used to validate the performance of residue analysis methods?
A) Internal standard
B) Solvent
C) Sample matrix
D) Calibration curve
Answer: A) Internal standard
28. What is a common method for extracting pesticide residues from food samples?
A) Solid-phase microextraction (SPME)
B) Liquid-liquid extraction (LLE)
C) Supercritical fluid extraction (SFE)
D) Ultra-high-pressure liquid chromatography (UHPLC)
Answer: B) Liquid-liquid extraction (LLE)
29. Which technique is used to determine the presence of chlorinated hydrocarbons in food?
A) Gas Chromatography (GC)
B) Nuclear Magnetic Resonance (NMR)
C) Ultraviolet-Visible Spectroscopy (UV-Vis)
D) Mass Spectrometry (MS)
Answer: A) Gas Chromatography (GC)
30. What is the function of a ‘spike’ in residue analysis?
A) To increase the sample volume
B) To test the accuracy of the analytical method
C) To improve the recovery rate
D) To measure the contamination level
Answer: B) To test the accuracy of the analytical method
31. Which method is used to measure the levels of heavy metals in food?
A) Atomic Absorption Spectroscopy (AAS)
B) Gas Chromatography (GC)
C) Enzyme-Linked Immunosorbent Assay (ELISA)
D) Nuclear Magnetic Resonance (NMR)
Answer: A) Atomic Absorption Spectroscopy (AAS)
32. What is the primary goal of using ‘blank’ and ‘spiked’ samples in residue analysis?
A) To increase sample volume
B) To ensure the accuracy and precision of the analysis
C) To reduce sample preparation time
D) To measure the environmental impact
Answer: B) To ensure the accuracy and precision of the analysis
33. Which contaminant is most commonly associated with agricultural products?
A) Heavy metals
B) Mycotoxins
C) Pesticides
D) Pathogens
Answer: C) Pesticides
34. What does ‘matrix effect’ refer to in residue analysis?
A) The effect of sample preparation on the analysis
B) The influence of the sample matrix on the analytical measurement
C) The calibration curve of the analytical method
D) The type of residue detected
Answer: B) The influence of the sample matrix on the analytical measurement
35. Which technique is used to analyze the presence of dioxins in food?
A) High-Resolution Mass Spectrometry (HRMS)
B) Gas Chromatography-Mass Spectrometry (GC-MS)
C) Liquid Chromatography-Mass Spectrometry (LC-MS)
D) Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
Answer: B) Gas Chromatography-Mass Spectrometry (GC-MS)
36. What is the purpose of using a ‘standard reference material’ in residue analysis?
A) To improve sample homogeneity
B) To calibrate analytical instruments
C) To validate the analytical method
D) To reduce sample preparation time
Answer: C) To validate the analytical method
37. Which method is most effective for the analysis of multiple pesticide residues simultaneously?
A) Gas Chromatography-Mass Spectrometry (GC-MS)
B) High-Performance Liquid Chromatography (HPLC)
C) Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
D) Enzyme-Linked Immunosorbent Assay (ELISA)
Answer: A) Gas Chromatography-Mass Spectrometry (GC-MS)
38. What type of contamination is usually detected using Mass Spectrometry (MS)?
A) Physical contaminants
B) Microbial contaminants
C) Chemical contaminants
D) Nutritional contaminants
Answer: C) Chemical contaminants
39. What is the main advantage of using Gas Chromatography-Mass Spectrometry (GC-MS) for residue analysis?
A) Simplicity of operation
B) Ability to detect a wide range of contaminants with high sensitivity
C) Low cost
D) Quick analysis time
Answer: B) Ability to detect a wide range of contaminants with high sensitivity
40. Which of the following is a common contaminant found in seafood?
A) Lead
B) Asbestos
C) Cadmium
D) Benzene
Answer: C) Cadmium
41. What does ‘accuracy’ refer to in residue analysis?
A) The consistency of the measurement
B) The closeness of the measured value to the true value
C) The sensitivity of the analytical method
D) The reproducibility of the analysis
Answer: B) The closeness of the measured value to the true value
42. Which method is commonly used for detecting trace levels of mycotoxins in food?
A) Gas Chromatography-Mass Spectrometry (GC-MS)
B) High-Performance Liquid Chromatography (HPLC)
C) Fourier Transform Infrared Spectroscopy (FTIR)
D) Ultraviolet-Visible Spectroscopy (UV-Vis)
Answer: B) High-Performance Liquid Chromatography (HPLC)
43. What is the function of a ‘standard addition’ method in residue analysis?
A) To measure the accuracy of the analytical method
B) To compensate for matrix effects
C) To improve sample preparation
D) To calibrate the analytical instrument
Answer: B) To compensate for matrix effects
44. Which contaminant is associated with the use of certain food packaging materials?
A) Phthalates
B) Mycotoxins
C) Pesticides
D) Heavy metals
Answer: A) Phthalates
45. What does ‘selectivity’ refer to in the context of residue analysis?
A) The ability to detect a specific contaminant among many
B) The overall sensitivity of the analytical method
C) The speed of the analysis
D) The cost of the analytical method
Answer: A) The ability to detect a specific contaminant among many
46. What is the purpose of a ‘calibration curve’ in residue analysis?
A) To measure the recovery rate of the contaminants
B) To determine the concentration of contaminants in the sample
C) To test the sample matrix effect
D) To evaluate the performance of the analytical method
Answer: B) To determine the concentration of contaminants in the sample
47. Which technique is used for the detection of radioactivity in food samples?
A) Liquid Scintillation Counting (LSC)
B) Gas Chromatography (GC)
C) Atomic Absorption Spectroscopy (AAS)
D) High-Performance Liquid Chromatography (HPLC)
Answer: A) Liquid Scintillation Counting (LSC)
48. What does ‘sensitivity’ refer to in residue analysis?
A) The ability to detect low levels of contaminants
B) The ability to separate different contaminants
C) The accuracy of the measurement
D) The speed of the analysis
Answer: A) The ability to detect low levels of contaminants
49. Which of the following is an example of a non-targeted analysis technique?
A) Gas Chromatography-Mass Spectrometry (GC-MS)
B) High-Performance Liquid Chromatography (HPLC)
C) Fourier Transform Infrared Spectroscopy (FTIR)
D) Liquid Chromatography-Mass Spectrometry (LC-MS)
Answer: C) Fourier Transform Infrared Spectroscopy (FTIR)
50. Which of the following contaminants is commonly associated with the use of agricultural chemicals?
A) Heavy metals
B) Mycotoxins
C) Pesticides
D) Pathogens
Answer: C) Pesticides
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