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Analytical techniques (chromatography, spectroscopy) MCQs Food science

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1. . What does chromatography primarily separate?





2. . Which of the following is a type of chromatography where the stationary phase is a liquid coated on a solid support?





3. . In gas chromatography (GC), what is typically used as the mobile phase?





4. . Which type of chromatography uses a liquid mobile phase and a solid stationary phase?





5. . What is the primary purpose of using a detector in chromatography?





6. . What does the term “retention time” refer to in chromatography?





7. . Which type of chromatography is commonly used for separating complex mixtures in food science?





8. . In high-performance liquid chromatography (HPLC), what is the role of the pump?





9. . Which type of detector in HPLC is based on measuring UV light absorption?





10. . What is the main principle of mass spectrometry (MS)?





11. . Which technique combines chromatography with mass spectrometry for detailed analysis of compounds?





12. . What does “spectroscopy” measure in analytical techniques?





13. . Which type of spectroscopy is used to identify functional groups in organic compounds?





14. . What is the primary use of ultraviolet-visible (UV-Vis) spectroscopy in food science?





15. . Which type of spectroscopy provides information about the molecular structure of compounds by analyzing their magnetic properties?





16. . What does the “chromatographic peak area” in a chromatogram indicate?





17. . Which type of chromatography is best suited for separating gases?





18. . What is the main advantage of using liquid chromatography (LC) over other chromatography techniques?





19. . Which of the following detectors in GC measures compounds based on their thermal conductivity?





20. . In UV-Vis spectroscopy, what does the absorbance value represent?





21. . What is the role of the stationary phase in chromatography?





22. . Which technique uses a silica gel or alumina plate as the stationary phase?





23. . What is the primary purpose of using a column in chromatography?





24. . Which technique is best for detecting trace amounts of compounds in complex mixtures?





25. . What is “resolution” in the context of chromatography?





26. . Which type of chromatography is used for separating proteins and peptides?





27. . In NMR spectroscopy, what information does the chemical shift provide?





28. . Which of the following is a key feature of high-performance liquid chromatography (HPLC)?





29. . What is the purpose of a calibration curve in spectroscopy?





30. . Which type of detector in HPLC is used for detecting compounds that do not absorb UV light?





31. . In gas chromatography, what is the purpose of the injector?





32. . Which type of spectroscopy provides information about the molecular vibrations and rotations of a compound?





33. . Which detector in GC is used to measure compounds that are not easily ionized?





34. . What is the primary benefit of using supercritical fluid chromatography (SFC)?





35. . What does “peak area” in chromatography represent?





36. . Which type of NMR spectroscopy provides information about the molecular dynamics and flexibility of compounds?





37. . Which of the following is an advantage of using UV-Vis spectroscopy?





38. . What is the function of the mobile phase in chromatography?





39. . Which chromatography technique is most suitable for analyzing volatile compounds?





40. . In chromatography, what does “selectivity” refer to?





41. . Which type of spectroscopy can provide information about the electronic structure of a molecule?





42. . What does “peak height” in a chromatogram typically indicate?





43. . Which technique uses a mobile phase that is supercritical fluid?





44. . In mass spectrometry, what does “molecular ion peak” represent?





45. . Which detector in HPLC is used for compounds that exhibit fluorescence?





46. . What is the primary purpose of using a gradient elution in HPLC?





47. . In IR spectroscopy, which region is typically used to identify functional groups?





48. . What does “chromatographic resolution” measure?





49. . Which chromatography technique is known for its high sensitivity and resolution?





50. . In UV-Vis spectroscopy, what does a higher absorbance value indicate?





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