Laboratory Techniques MCQs

1. Which laboratory technique is used to separate proteins based on their size and charge?
A) Polymerase Chain Reaction (PCR)
B) Western Blotting
C) Gel Electrophoresis
D) Chromatography

Answer: C) Gel Electrophoresis

2. What is the purpose of a spectrophotometer in a laboratory?
A) To measure pH levels
B) To determine the concentration of substances based on light absorption
C) To analyze DNA sequences
D) To perform titrations

Answer: B) To determine the concentration of substances based on light absorption

3. Which technique is used to amplify DNA fragments in the laboratory?
A) Polymerase Chain Reaction (PCR)
B) ELISA (Enzyme-Linked Immunosorbent Assay)
C) Immunohistochemistry
D) Southern Blotting

Answer: A) Polymerase Chain Reaction (PCR)

4. What is the purpose of a fume hood in a laboratory?
A) To store chemicals safely
B) To provide a controlled environment for sensitive experiments
C) To prevent exposure to hazardous fumes
D) To maintain a constant temperature

Answer: C) To prevent exposure to hazardous fumes

5. Which laboratory technique is used to separate and analyze DNA fragments based on their size?
A) ELISA
B) PCR
C) Southern Blotting
D) Agarose Gel Electrophoresis

Answer: D) Agarose Gel Electrophoresis

6. What is the primary purpose of a centrifuge in a laboratory?
A) To measure the density of liquids
B) To mix reagents thoroughly
C) To separate substances based on density or mass
D) To sterilize equipment

Answer: C) To separate substances based on density or mass

7. Which laboratory technique is used to visualize the internal structure of cells or tissues?
A) Immunohistochemistry
B) PCR
C) Titration
D) NMR Spectroscopy

Answer: A) Immunohistochemistry

8. What is the role of a pipette in laboratory work?
A) To measure temperature
B) To measure volume and transfer liquids accurately
C) To analyze gas concentrations
D) To perform titrations

Answer: B) To measure volume and transfer liquids accurately

9. Which laboratory technique is used to separate and purify proteins based on their size, charge, and affinity?
A) Chromatography
B) PCR
C) ELISA
D) Gel Electrophoresis

Answer: A) Chromatography

10. What is the purpose of a Bunsen burner in a laboratory?
A) To measure pH levels
B) To provide a source of heat for sterilization and heating solutions
C) To analyze DNA sequences
D) To perform titrations

Answer: B) To provide a source of heat for sterilization and heating solutions

11. Which laboratory technique is used to detect and measure specific proteins or antibodies in a sample?
A) Southern Blotting
B) PCR
C) ELISA
D) Western Blotting

Answer: C) ELISA (Enzyme-Linked Immunosorbent Assay)

12. What is the purpose of a laminar flow hood in a laboratory?
A) To store chemicals safely
B) To provide a sterile environment for sensitive experiments
C) To measure light absorption
D) To perform titrations

Answer: B) To provide a sterile environment for sensitive experiments

13. Which laboratory technique is used to study the structure and interactions of molecules?
A) Gel Electrophoresis
B) X-ray Crystallography
C) PCR
D) ELISA

Answer: B) X-ray Crystallography

14. What is the role of a microtome in laboratory work?
A) To measure volume
B) To perform titrations
C) To cut thin sections of tissues for microscopic analysis
D) To analyze gas concentrations

Answer: C) To cut thin sections of tissues for microscopic analysis

15. Which laboratory technique is used to identify and quantify the elements present in a sample?
A) PCR
B) NMR Spectroscopy
C) Gel Electrophoresis
D) Titration

Answer: B) NMR Spectroscopy (Nuclear Magnetic Resonance Spectroscopy)

16. What is the purpose of a pH meter in a laboratory?
A) To measure temperature
B) To measure acidity or alkalinity of solutions
C) To analyze DNA sequences
D) To perform titrations

Answer: B) To measure acidity or alkalinity of solutions

17. Which laboratory technique is used to determine the concentration of a substance in a solution by reacting it with a known solution?
A) Chromatography
B) PCR
C) Titration
D) ELISA

Answer: C) Titration

18. What is the role of a petri dish in laboratory experiments?
A) To measure volume
B) To perform titrations
C) To culture microorganisms or cells
D) To analyze gas concentrations

Answer: C) To culture microorganisms or cells

19. Which laboratory technique is used to analyze the structure and composition of organic compounds?
A) Chromatography
B) Mass Spectrometry
C) Titration
D) Gel Electrophoresis

Answer: B) Mass Spectrometry

20. What is the purpose of a water bath in a laboratory?
A) To measure temperature
B) To measure acidity or alkalinity
C) To analyze DNA sequences
D) To perform titrations

Answer: A) To measure temperature

21. Which laboratory technique is used to separate and analyze complex mixtures of compounds based on their physical and chemical properties?
A) PCR
B) Chromatography
C) ELISA
D) NMR Spectroscopy

Answer: B) Chromatography

22. What is the role of a balance in laboratory work?
A) To measure volume
B) To measure mass or weight accurately
C) To analyze gas concentrations
D) To perform titrations

Answer: B) To measure mass or weight accurately

23. Which laboratory technique is used to study the interaction between antigens and antibodies?
A) PCR
B) Western Blotting
C) ELISA
D) Gel Electrophoresis

Answer: B) Western Blotting

24. What is the purpose of a hot plate in a laboratory?
A) To measure temperature
B) To measure acidity or alkalinity
C) To analyze DNA sequences
D) To provide a source of heat for experiments

Answer: D) To provide a source of heat for experiments

25. Which laboratory technique is used to study the three-dimensional structure of proteins?
A) Chromatography
B) Mass Spectrometry
C) X-ray Crystallography
D) NMR Spectroscopy

Answer: C) X-ray Crystallography

26. What is the role of a desiccator in laboratory experiments?
A) To measure temperature
B) To provide a controlled environment for reactions
C) To analyze gas concentrations
D) To remove moisture from samples

Answer: D) To remove moisture from samples

27. Which laboratory technique is used to separate and identify components of a mixture based on their boiling points?
A) Chromatography
B) Distillation
C) PCR
D) ELISA

Answer: B) Distillation

28. What is the purpose of a magnetic stirrer in a laboratory?
A) To measure volume
B) To mix solutions using a magnetic field
C) To analyze gas concentrations
D) To perform titrations

Answer: B) To mix solutions using a magnetic field

29. Which laboratory technique is used to measure the absorption of light by molecules in a sample?
A) PCR
B) Spectrophotometry
C) ELISA
D) Gel Electrophoresis

Answer: B) Spectrophotometry

30. What is the role of a centrifugal evaporator in laboratory work?
A) To measure temperature
B) To evaporate solvents from samples using centrifugal force
C) To analyze gas concentrations
D) To perform titrations

Answer: B) To evaporate solvents from samples using centrifugal force

31. Which laboratory technique is used to study the physical and chemical properties of materials at the nanoscale?
A) Nanodrop Spectrophotometry
B) Nanoparticle Analysis
C) Scanning Electron Microscopy (SEM)
D) Gas Chromatography

Answer: C) Scanning Electron Microscopy (SEM)

32. What is the purpose of a pH indicator in laboratory experiments?
A) To measure temperature
B) To measure acidity or alkalinity
C) To analyze DNA sequences
D) To perform titrations

Answer: B) To measure acidity or alkalinity

33. Which laboratory technique is used to study the structure and function of biomolecules within cells?
A) Electron Microscopy
B) Cell Culture
C) PCR
D) ELISA

Answer: A) Electron Microscopy

34. What is the role of a vortex mixer in laboratory work?
A) To measure volume
B) To mix small volumes of liquids rapidly
C) To analyze gas concentrations
D) To perform titrations

Answer: B) To mix small volumes of liquids rapidly

35. Which laboratory technique is used to separate and analyze complex mixtures of proteins based on their size and charge?
A) Gel Electrophoresis
B) Chromatography
C) Mass Spectrometry
D) PCR

Answer: A) Gel Electrophoresis

36. What is the purpose of a centrifuge tube in laboratory experiments?
A) To measure temperature
B) To hold samples during centrifugation
C) To analyze DNA sequences
D) To perform titrations

Answer: B) To hold samples during centrifugation

37. Which laboratory technique is used to study the interaction between proteins and nucleic acids?
A) ELISA
B) Western Blotting
C) Co-immunoprecipitation
D) PCR

Answer: C) Co-immunoprecipitation

38. What is the role of a cryostat in laboratory work?
A) To measure temperature
B) To maintain low temperatures for tissue sectioning
C) To analyze gas concentrations
D) To perform titrations

Answer: B) To maintain low temperatures for tissue sectioning

39. Which laboratory technique is used to analyze the sequence of nucleotides in DNA?
A) PCR
B) Gel Electrophoresis
C) DNA Sequencing
D) ELISA

Answer: C) DNA Sequencing

40. What is the purpose of a bacterial incubator in a laboratory?
A) To measure temperature
B) To provide a controlled environment for bacterial growth
C) To analyze gas concentrations
D) To perform titrations

Answer: B) To provide a controlled environment for bacterial growth

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