Genetic Engineering MCQs

1. What is genetic engineering?
A) The study of hereditary traits
B) The process of modifying an organism’s genetic material
C) The study of DNA replication
D) The production of enzymes
Answer: B) The process of modifying an organism’s genetic material

2. Which enzyme is commonly used to cut DNA at specific sequences in genetic engineering?
A) DNA polymerase
B) RNA polymerase
C) Restriction enzyme
D) Ligase
Answer: C) Restriction enzyme

3. What is the purpose of a vector in genetic engineering?
A) To amplify DNA
B) To cut DNA
C) To carry foreign DNA into a host cell
D) To synthesize proteins
Answer: C) To carry foreign DNA into a host cell

4. Which of the following is NOT a commonly used vector in genetic engineering?
A) Plasmid
B) Bacteriophage
C) Virus
D) Chromosome
Answer: D) Chromosome

5. What is the role of a selectable marker gene in genetic engineering?
A) To amplify DNA
B) To cut DNA
C) To identify transformed cells
D) To synthesize proteins
Answer: C) To identify transformed cells

6. Which of the following is a type of genetic modification commonly used in plants?
A) Cloning
B) Gene editing
C) Polymerase chain reaction (PCR)
D) DNA sequencing
Answer: B) Gene editing

7. What is the function of CRISPR-Cas9 in genetic engineering?
A) To amplify DNA
B) To cut DNA at specific sequences
C) To synthesize proteins
D) To replicate DNA
Answer: B) To cut DNA at specific sequences

8. Which technique is used to introduce foreign DNA into animal cells in genetic engineering?
A) Electroporation
B) Polymerase chain reaction (PCR)
C) Gel electrophoresis
D) Southern blotting
Answer: A) Electroporation

9. What is the purpose of gene cloning in genetic engineering?
A) To produce genetically identical organisms
B) To amplify DNA
C) To analyze gene expression
D) To study DNA replication
Answer: B) To amplify DNA

10. Which of the following is an example of a genetically modified organism (GMO)?
A) A mouse with blue eyes
B) A tomato with increased shelf life
C) A bird with longer wings
D) A fish with a different habitat
Answer: B) A tomato with increased shelf life

11. What is the function of a promoter in genetic engineering?
A) To terminate gene expression
B) To signal the start of gene transcription
C) To replicate DNA
D) To produce proteins
Answer: B) To signal the start of gene transcription

12. What is the significance of gene editing technologies like CRISPR-Cas9 in genetic engineering?
A) They allow precise modification of DNA sequences
B) They produce genetically identical organisms
C) They amplify DNA
D) They terminate gene expression
Answer: A) They allow precise modification of DNA sequences

13. Which of the following is NOT a potential application of genetic engineering in agriculture?
A) Increasing crop yield
B) Enhancing nutritional content
C) Producing new plant species
D) Creating drought-resistant plants
Answer: C) Producing new plant species

14. What is the purpose of a reporter gene in genetic engineering?
A) To replicate DNA
B) To identify transformed cells
C) To synthesize proteins
D) To terminate gene expression
Answer: B) To identify transformed cells

15. What is a transgenic organism in genetic engineering?
A) An organism with a modified genome
B) An organism with a shorter lifespan
C) An organism with a larger habitat
D) An organism with a reduced reproductive rate
Answer: A) An organism with a modified genome

16. Which of the following is a technique used to analyze the expression of specific genes in genetic engineering?
A) Southern blotting
B) Northern blotting
C) Western blotting
D) Gel electrophoresis
Answer: B) Northern blotting

17. What is the function of a terminator sequence in genetic engineering?
A) To amplify DNA
B) To signal the end of gene transcription
C) To produce proteins
D) To cut DNA
Answer: B) To signal the end of gene transcription

18. Which of the following is an example of gene therapy?
A) Cloning a gene
B) Editing a gene in a patient’s cells to treat a genetic disorder
C) Studying gene expression
D) Producing genetically modified organisms
Answer: B) Editing a gene in a patient’s cells to treat a genetic disorder

19. What is the function of a transposon in genetic engineering?
A) To replicate DNA
B) To move genes within a genome
C) To terminate gene expression
D) To amplify DNA
Answer: B) To move genes within a genome

20. Which technique is used to separate DNA fragments based on size in genetic engineering?
A) Polymerase chain reaction (PCR)
B) Southern blotting
C) Gel electrophoresis
D) Northern blotting
Answer: C) Gel electrophoresis

21. What is the purpose of gene knockout in genetic engineering?
A) To enhance gene expression
B) To replicate DNA
C) To remove or deactivate a gene of interest
D) To terminate gene expression
Answer: C) To remove or deactivate a gene of interest

22. Which of the following is a potential benefit of genetic engineering in medicine?
A) Increased biodiversity
B) Development of new antibiotics
C) Enhanced crop yield
D) Treatment of genetic disorders
Answer: D) Treatment of genetic disorders

23. What is a restriction site in genetic engineering?
A) A site where DNA replication occurs
B) A specific DNA sequence recognized by a restriction enzyme
C) A region of DNA that codes for proteins
D) A site for gene transcription
Answer: B) A specific DNA sequence recognized by a restriction enzyme

24. What is the role of a plasmid in genetic engineering?
A) To replicate DNA
B) To carry foreign DNA into a host cell
C) To produce proteins
D) To cut DNA
Answer: B) To carry foreign DNA into a host cell

25. Which of the following is an example of a genetically modified (GM) crop?
A) An apple with a different color
B) A tomato with increased resistance to pests
C) A banana with a longer shelf life
D) A strawberry with a different taste
Answer: B) A tomato with increased resistance to pests

26. What is genetic engineering?
A) A process of manipulating genes
B) A process of studying fossils
C) A process of breeding animals naturally
D) A process of creating new ecosystems
Answer: A) A process of manipulating genes

27. Which of the following is NOT a tool used in genetic engineering?
A) PCR
B) CRISPR-Cas9
C) MRI
D) Restriction enzymes
Answer: C) MRI

28. What is the function of restriction enzymes in genetic engineering?
A) To amplify DNA
B) To cut DNA at specific sequences
C) To insert genes into cells
D) To analyze DNA sequences
Answer: B) To cut DNA at specific sequences

29. What is the purpose of a vector in genetic engineering?
A) To analyze DNA
B) To cut DNA
C) To carry foreign DNA into host cells
D) To amplify DNA
Answer: C) To carry foreign DNA into host cells

30. Which of the following is an example of a genetically modified organism (GMO)?
A) A cat with blue eyes
B) A tomato with increased shelf life
C) A dog with longer fur
D) A horse with faster speed
Answer: B) A tomato with increased shelf life

31. What is the role of DNA ligase in genetic engineering?
A) To cut DNA
B) To amplify DNA
C) To join DNA fragments
D) To analyze DNA sequences
Answer: C) To join DNA fragments

32. What does CRISPR-Cas9 technology do in genetic engineering?
A) It cuts DNA at specific sequences
B) It amplifies DNA
C) It analyzes DNA
D) It inserts genes into cells
Answer: A) It cuts DNA at specific sequences

33. Which of the following is a potential application of genetic engineering in agriculture?
A) Creating new planets
B) Producing drought-resistant crops
C) Generating electricity
D) Curing cancer
Answer: B) Producing drought-resistant crops

34. What is gene editing in the context of genetic engineering?
A) Studying genetic variations
B) Replacing an existing gene with a modified one
C) Analyzing gene expressions
D) Cloning animals
Answer: B) Replacing an existing gene with a modified one

35. What is a transgenic organism?
A) An organism with multiple legs
B) An organism with genes from another species
C) An organism with no genetic material
D) An organism with wings
Answer: B) An organism with genes from another species

36. What is gene therapy?
A) Modifying genes in plants
B) Correcting genetic defects in humans
C) Creating new species
D) Increasing the lifespan of animals
Answer: B) Correcting genetic defects in humans

37. Which technique allows scientists to create multiple copies of a specific DNA segment?
A) PCR
B) MRI
C) X-ray
D) Gene therapy
Answer: A) PCR

38. What is the primary benefit of genetically modified crops?
A) They require more water
B) They are resistant to pests
C) They have shorter lifespans
D) They produce less yield
Answer: B) They are resistant to pests

39. What is the significance of the Human Genome Project in genetic engineering?
A) It discovered new planets
B) It mapped the entire human genome
C) It cloned dinosaurs
D) It created new species
Answer: B) It mapped the entire human genome

40. What ethical concerns are associated with genetic engineering in humans?
A) Creating superhumans
B) Curing genetic diseases
C) Enhancing intelligence
D) All of the above
Answer: D) All of the above

41. Which enzyme is commonly used to insert foreign DNA into a plasmid during genetic engineering?
A) DNA polymerase
B) RNA polymerase
C) Ligase
D) Restriction enzyme
Answer: C) Ligase

42. Which of the following is an example of gene cloning?
A) Creating identical twins
B) Crossing two different species
C) Inserting a gene from one organism into another
D) Splicing DNA
Answer: C) Inserting a gene from one organism into another

43. What is the role of a promoter in genetic engineering?
A) It cuts DNA
B) It amplifies DNA
C) It initiates gene expression
D) It joins DNA fragments
Answer: C) It initiates gene expression

44. What is synthetic biology?
A) Studying natural biological systems
B) Creating artificial life forms
C) Analyzing DNA sequences
D) Cloning animals
Answer: B) Creating artificial life forms

45. Which of the following is a potential application of genetic engineering in medicine?
A) Producing new smartphones
B) Curing genetic disorders
C) Creating new clothing materials
D) Building skyscrapers
Answer: B) Curing genetic disorders

46. What is the role of a transposon in genetic engineering?
A) It cuts DNA
B) It amplifies DNA
C) It moves genes within a genome
D) It joins DNA fragments
Answer: C) It moves genes within a genome

47. Which enzyme is responsible for making complementary RNA from DNA during genetic engineering?
A) RNA polymerase
B) DNA polymerase
C) Ligase
D) Restriction enzyme
Answer: A) RNA polymerase

48. What is the purpose of gene knockout in genetic engineering?
A) To insert foreign genes
B) To deactivate a specific gene
C) To clone organisms
D) To amplify DNA
Answer: B) To deactivate a specific gene

49. What is the significance of CRISPR technology in genetic engineering?
A) It allows precise gene editing
B) It amplifies DNA
C) It analyzes DNA sequences
D) It clones organisms
Answer: A) It allows precise gene editing

50. What is gene silencing in genetic engineering?
A) Activating a gene
B) Deactivating a gene
C) Cloning a gene
D) Amplifying a gene
Answer: B) Deactivating a gene

51. Which of the following is a potential environmental impact of genetically modified organisms (GMOs)?
A) Increased biodiversity
B) Reduced pesticide use
C) Spread of transgenes to wild populations
D) Higher water consumption
Answer: C) Spread of transgenes to wild populations

Genetic Engineering MCQs

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